

Monitoring comprises confirming that the pressure, temperature, and exposure duration have reached the levels advised by the steriliser manufacturer and recording this information in your sterilisation records.

With each steriliser load, mechanical and chemical monitoring should be performed. While mechanical and chemical indicators do not ensure sterilisation, they do aid in identifying operational mistakes (such as overloaded steriliser or improper packaging) and equipment problems. The choice of bacterial spores for a method also considers the spores' known resistance to that method.įor instance, Geobacillus stearothermophilus spores are used in BIs that monitor these sterilising processes because they are very resistant to steam and vaporised hydrogen peroxide. Endospores or bacterial spores are the microorganisms most usually used in BIs. A biological indicator provides data on whether the conditions were satisfied for a sterilisasterilisation procedure eliminate a certain number of germs, demonstrating pprocess'sconfidence. Biological Indicators: What Are They?īiological indicators (BIs), according to ANSI/AAMI and ISO, are test items containing live microorganisms that demonstrate a specific level of resistance to a particular sterilising process. The most widely used method of sterilisation monitoring is biological indicators, or spore tests, which evaluate the sterilising process directly by eradicating well-known, highly resistant microorganisms (such as Bacillus or Bacillus species).
